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Process optimization for amyloglucosidase by a mutant strain of Aspergillus niger in stirred fermenter
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نویسنده
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malik s. ,iftikhar t. ,haq i.u. ,khattak m.i.
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منبع
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pakistan journal of botany - 2013 - دوره : 45 - شماره : 2 - صفحه:663 -666
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چکیده
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The present study was designed to optimize the process parameters for the production of amyloglucosidase bya mutant strain of aspergillus niger in stirred fermenter by. for this purpose,various cultural conditions like rate of fermentation,process ph,rate of agitation and size of inoculum was investigated. the maximum production (25.15 u/ml/min) of amyloglucosidase was achieved at the agitation speed of 200rpm. the production of amyloglucosidase was found to be maximum (25.08 u/ml/min) at ph 5 of the medium. the optimum productivity (25.15 u/ml/min) of the enzyme was achieved with 4% inoculum after 48 h of incubation. the process temperature was optimized at 30°c throughout the study.
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آدرس
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institute of industrial biotechnology,gc university,lahore,pakistan,department of botany,queen mary college, Pakistan, department of botany,govt. college university, Pakistan, institute of industrial biotechnology,gc university, Pakistan, chemistry department,balochistan university, Pakistan
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Authors
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