Function analysis of phytochrome B gene in maize

Zmphyb1 and zmphyb2 with some other factors and their own interactions were studied using yeast two-hybrid technology. results showed that interaction signals were detected among different segments. results speculated that interaction signals between zmphyb1 and zmphyb2 could be detected under certain lighting condition after a certain period of time. atspa1 and atcop1 may form complexes with other interaction factors co-interacting zmphyb accumulation. agrobacterium-mediated genetic transformation study of functional zmphyb1 and zmphyb2 into arabidopsis thaliana showed that genes can complement the corresponding mutant phenotype,while over-expressing transgenic plants gave rise to more obvious phenotypes in photo-morphogenesis. we speculated that endogenous phyb gene interferences were responsible for different of indexes of necessary light-inducing phenotypic conditions between over-expression transform-ants and complementary transform-ants.