Hemato-biochemical and testicular changes induced by subchronic doses of triazophos in male Japanese quail

The present study was conducted to investigate the possible hemato-biochemical and histopathological changes induced by concurrent administration of triazophos in male japanese quail. for this purpose,healthy and sexually mature 60 adult male japanese quail were divided into five equal groups. the birds were kept in wire cages for six days for adaptation. the duration of the experiment was 48 days. all the birds in groups a-e were orally administered triazophos (o,o-diethyl o- 1phenyl-1 h-1,2,4- triazol-3-yl phosphorothioate) an organophosphate insecticide @ 0,2,4,6 and 8 mg/kg bw daily for 48 days. the blood/serum and morbid tissues were collected at post treatment days 16,32 and 48. significant (p ≤ 0.05) decrease in relative weight of testes and liver,while an increase in relative weight of thymus was recorded in treated birds as compared to controls. histologically,pyknotic nuclei with extensive vacuolation of hepatocytes and congestion in thymus was observed. treatment also induced various testicular changes including admixture of necrotic cells,arrest of spermatogenesis,decreased seminiferous tubule diameter,germinal epithelial height and number of seminiferous tubules with normal sperms. significantly increased activity of serum alanine transaminase,aspartate transaminase and lactate dehydrogenase with increased levels of cholesterol,triglycerides,high density lipids,low density lipids and malondialdehyde were also recorded in triazophos treated birds. moreover,frequency of micronuclei formation,erythrocytes with lobed nuclei,blebbed nuclei,notched nuclei and pear shaped erythrocytes was significantly increased in treated birds. the present study revealed for the first time that triazophos induces histo-architectural and serum biochemical changes in birds. © 2015 university of agriculture. all rights reserved.