Evaluation of commercial elisa kit for diagnosis of small ruminant fascioliasis in Pakistan

Elisa is a serological assay claimed for higher sensitivity and specificity in diagnosing sub-clinical fascioliasis. the purpose of this investigation was to evaluate the sensitivity and specificity of an elisa kit (drg ®,germany) for the diagnosis of small ruminant fascioliasis in district sargodha,punjab,pakistan. microscopically,226 animals out of 600 (37.66%) were found positive for fascioliasis. higher prevalence (38.78%; 121/312) was found in goats as compared to sheep (36.46%; 105/288). prevalence of fasciola hepatica was significantly higher as compared to fasciola gigantica (p<0.005). commercial elisa kit detected anti-fasciola antibodies in higher percentage in sheep and goat population as compared to coprological examination. seroprevalence was 48.61 and 49.36% in sheep and goat population. commercial elisa kit was more specific than coprological examination when compared in win episcope by screening of comparative diagnostics. © 2015,university of agriculture. all right reserved.