Genetic variability in dengue mosquito aedes aegypti (diptera: Culicidae) from punjab,Pakistan using rapd markers

The mosquito aedes aegypti is the main vector of dengue virus. although,the epidemiology of dengue fever has been widely described; however,the genetics of the vector mosquito remained poorly understood in pakistan. hence,this study was performed to estimate the genetic variability in ae. aegypti populations from different areas of the punjab,ae. aegypti mosquitoes were collected by using standard techniques (dippers) from faisalabad,nankana sahib and lahore districts. the collected populations were reared separately in the laboratory and then dna was extracted from single individual. ten decamer random primers (gl decamer a-01 to a-10) were selected for polymerase chain reaction (pcr). the pcr products were analyzed using 1.5% agarose gel electrophoresis. the amplification profile data showed monomorphism and polymorphism between different districts as well as between samples of even same district. the primer a-04 showed a clear polymorphic banding pattern in different samples from nankana sahib,faisalabad and lahore. maximum number of bands were produced by a-010 primer (75%) followed by a-02 (67%). the allele and genotype frequencies were measured at each locus. the nm value showed a migration between nankana sahib and faisalabad (4.65). while,ae. aegypti were freely moving within district. however,the populations were also slightly genetically different (heterozygosity; ht: 029). the dendrogram based on genetic similarities revealed three clusters which showed that ae. aegypti populations from three districts were slightly different from each other. the genetic similarity ranged from maximum 3.16 to minimum 1.00. based on the inter- and intra-populations polymorphic data from all three districts,it was concluded that ae. aegypti populations were slightly genetically different in selected areas. © 2015,university of agriculture. all rights reserved.