Rapd based estimation of genetic diversity in walnut genotypes growing in malakund division,Pakistan

Rapd based estimation of genetic diversity in walnut genotypes growing in malakund division,pakistan,was made by collecting nut samples of 226 walnut genotypes from four walnut growing districts of malakand division. on the basis of various qualitative traits,18 superior walnut genotypes were selected along with two exotic varieties. every single band was considered as a single locus/allele for all the genetic analyses. the 18 indigenous genotypes showed various levels of genetic polymorphism for the loci detected by using primers gla-05 and gla-10. the loci were scored as present (1) and absent (0). bivariate 1-0 data matrix was used to estimate genetic distances (gd) and for construction of phylogenetic tree based on cluster analysis. maximum genetic distance (100%) was observed between payne and sw-58 and payne and serr,closely followed by 94% dissimilarity between payne and dr-67. based on the data presented as dendrogram,the walnut genotypes were classified in to four major groups. the maximum numbers (13) of genotypes fall in group-i. sw-58 was the only genotypes in group-iv and the most distinct from others. classification of wild genotypes will help in breeding as well as vegetative propagation of walnut trees. © 2015,university of agriculture. all rights reserved.