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Alterations of DNA methylation in diverse grafted hybrid tomatoes (Solanum lycopersicum L.)
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نویسنده
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xincheng x. ,ali z. ,weiyi s. ,ghori n. ,hongbo s. ,jinping d.
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منبع
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pakistan journal of agricultural sciences - 2016 - دوره : 53 - شماره : 1 - صفحه:107 -112
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چکیده
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Tomato is an edible and useful vegetable. navel rot disease and low light stress are the problem in tomato cultivation. tomato breeding is a potential cheap solution but takes longer time. grafting technology provides instant solution like cultivating sensitive tomato (scion) grafted on resistant rootstock on non-optimal environmental conditions. dna methylation of grafted plants might help to adapt to the adverse conditions and has become a hot point in researches concerning vegetable breeding. in this research,disease-resistant tomato cultivar “1221” was used as rootstock,while disease-sensitive tomato cultivar “money maker (mm)” was used as scion. after grafting,changes of genomic global dna methylation were investigated through msap analysis. according to msap analysis,alterations of degree of dna methylation patterns were observed,which might help graft hybrids to adapt in response to the internal and external stimuli. © 2016,university of agriculture. all rights reserved.
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کلیدواژه
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MSAP analysis; PAGE; PCR; Restriction digestion; Silver staining
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آدرس
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department of life science,shangqiu normal university,shangqiu, China, department of plant breeding and genetics,university of agriculture,faisalabad,pakistan,department of plant breeding and genetics,mns university of agriculture,multan, Pakistan, department of life science,shangqiu normal university,shangqiu, China, department of plant breeding and genetics,university of agriculture,faisalabad, Pakistan, institute of agro-biotechnology,jiangsu academy of agricultural sciences,nanjing,china,key laboratory of coastal biology and bioresources utilization,yantai institute of coastal zone research (yic),chinese academy of sciences (cas),yantai, China, department of life science,shangqiu normal university,shangqiu, China
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Authors
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