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Single nucleotide polymorphisms in DRB1,IGF1 and ILs associated with fecal egg count confers resistance against Haemonchus contortus infection in goats
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نویسنده
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asif a.r. ,qadri s. ,fu y. ,alim m.a. ,wu z. ,ijaz n. ,cao j. ,javed r. ,ahmed s. ,awais m. ,ansari a.r. ,du x.
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منبع
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pakistan journal of agricultural sciences - 2016 - دوره : 53 - شماره : 4 - صفحه:963 -970
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چکیده
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Haemonchus contortus is a blood sucking gastrointestinal (gi) parasite that causes heavy production losses in small ruminants due to their poor immune response and rapid development of anthelmentic resistance. identifying genes involving in immune related pathways against gi nematode infection will assist in better responding and curing the ill effects of infection with h. contortus. fecal egg counts and ear tissues were collected from 200 goats belonging to four breeds and pcr sequencing method was used for identification of 25 seven single nucleotide polymorphisms (snps) and found two snps of dopamine receptor binding 1 (drb-1) (37 c/g; 10375 c/t),one from insulin-like growth factor1 (igf-1) (58110 c/g),three from interleukin (il) 32 (7638 g/c; 9375 a/g; 1158 a/g) and one from il-33 (70589 c/a) significantly (p<0.02) associated with fecal egg count (fec). out of those seven snps,5 were missense and changed the amino acids from (pro leu) in drb1,il32 (arg trp and ile val) and in il33 (arg trp). these missense snps may change the function of genes which play a crucial rule in immunity of host and expulsion of gi nematode. models of the snps of drb-1,il-32,il-33 and igf-1 genes were found significant (p<0.02) indicating a strong association with fec of h. contortous in goat. the haplotypes ccc and gct of drb-1,aag,agg,ggc,gca,and ggg of il-32 gene were found significantly (p≤0.03) associated with fec. the results of linkage disequilibrium (ld) showed that most of the snps were in the range of (0.29-0.98). therefore,the present study investigated the role of these candidate gene snps in innate immunity and concluded that these snps were potentially important for future goat breeding plans against h. contortus infection in goats. © 2016,university of agriculture,all rights reserved.
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کلیدواژه
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Association analysis; Goat; Immune response; Interleukins; Single nucleotide polymorphisms
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آدرس
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key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan,china,department of theriogenology,college of veterinary and animal sciences (cvas),jhang,pakistan,university of veterinary and animal sciences,lahore, Pakistan, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,department of theriogenology,college of veterinary and animal sciences (cvas),jhang,pakistan,university of veterinary and animal sciences,lahore, Pakistan, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan, China, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan, China, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan, China, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan, China, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan, China, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan, China, college of informatics,huazhong agricultural university,wuhan, China, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan, China, department of anatomy,histology and embryology,college of animal and veterinary medicine,huazhong agricultural university,wuhan,china,department of theriogenology,college of veterinary and animal sciences (cvas),jhang,pakistan,university of veterinary and animal sciences,lahore, Pakistan, key lab of animal genetics,breeding and reproduction,ministry education,college of animal science and technology,huazhong agricultural university,wuhan,china,the cooperative innovation center for sustainable pig production,wuhan,china,college of informatics,huazhong agricultural university,wuhan, China
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Authors
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