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ANTHER CULTURE EFFECTIVENESS IN PRODUCING DOUBLED HAPLOIDS OF CEREALS
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نویسنده
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Grauda Dace ,Mikelsone Andra ,Lisina Nadezda ,Zagata Karlis ,Ornicans Reinis ,Fokina Oksana ,Lapina Lita ,Rashal Isaak
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منبع
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proceedings of the latvian academy of sciences section b natural exact and applied sciences - 2014 - دوره : 68 - شماره : 3&4 - صفحه:142 -147
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چکیده
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Our goal was to improve the method of obtaining double haploids (dh) by anther culture from latvian breeding material for several agriculturally important cereals in latvia: barley, spring and winter wheat. hybrids from latvian breeders were initial material. it was found that copper (2.5 mg/l) added to barley anther pre-treatment media and to both barley (c3) and wheat (liquid amc) induction media, resulted in a higher percentage of green plants-regenerants. in general, the winter wheat hybrids exhibited lower embryogenesis than spring wheat hybrids. obtaining a large number of dh lines from barley and wheat hybrids with unknown androgenesis response can be organized in two stages: first — selection from breeding initial material hybrids responsive in anther culture, which enables production of green plants-regenerants, and second — production of dh lines in a large scale from selected hybrids.
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کلیدواژه
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DH lines ,breeding ,barley ,wheat ,copper
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آدرس
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University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA
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پست الکترونیکی
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izaks@email.lubi.edu.lv
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Authors
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