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REACTION OF FLAX (Linum usitatissimum L.) CALLI CULTURE TO SUPPLEMENT OF MEDIUM BY CARBON NANOPARTICLES
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نویسنده
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Kokina Inese ,Sledevskis Eriks ,Gerbreders Vjaceslavs ,Grauda Dace ,Jermalonoka Marija ,Valaine Kristina ,Gavarane Inese ,Piginka Inga ,Filipovics Maksims ,Rashal Isaak
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منبع
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proceedings of the latvian academy of sciences section b natural exact and applied sciences - 2012 - دوره : 66 - شماره : 4&5 - صفحه:200 -209
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چکیده
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Frequency of callusogenesis, type and capacity of regeneration, cell ploidy, dna methylation level, histological features, autofluorescence and photobleaching effects were studied in flax calli grown on medium supplemented with different concentrations of carbon (c) nanoparticles. the presence of carbon nanoparticles on medium decreased the percentage calli formation. detailed histological study led to better understanding of changes in calli caused by nanoparticles. ploidy variation in calli cells significantly depended on carbon nanoparticle concentration in the medium. different types of calli regeneration associated with different c nanoparticles concentration in medium were observed: somatic embryogenesis (direct or indirect) or organogenesis. c nanoparticle dose-dependent dna hypermethylation in flax calli cells were found. increased repair ability during laser irradiation was found in calli grown on medium with carbon nanoparticles.
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کلیدواژه
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flax ,calli ,cell ploidy ,DNA methylation ,somatic embryo ,histology ,autofluorescence ,photobleaching
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آدرس
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Daugavpils University, Institute of Systematic Biology, LATVIA, Daugavpils University, G Liberts' Innovative Microscopy Centre, LATVIA, Daugavpils University, G Liberts' Innovative Microscopy Centre, LATVIA, University of Latvia, Institute of Biology, LATVIA, Daugavpils University, Institute of Systematic Biology, LATVIA, Daugavpils University, Institute of Systematic Biology, LATVIA, Daugavpils University, Institute of Systematic Biology, LATVIA, Institute of Food Safety, Animal Health and Environment “BIOR”, LATVIA, Daugavpils University, Institute of Systematic Biology, LATVIA, University of Latvia, Institute of Biology, LATVIA
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Authors
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