ALLOGENEIC BONE MARROW MULTIPOTENT MESENCHYMAL STROMAL CELLS AND POLYTRAUMA REPAIR: THE ROLE OF FRACTIONATED ON THE BASIS OF MOLECULAR MASS RED BEETROOT JUICE IN THE PREVENTION OF TRANSPLANTED CELLS S

Red beetroot (beta vulgaris) juice (rbj) is used as a traditional medicine for treatment of anemia.it has been shown that beetroot juice decreases blood pressure, provides a protective effect onblood vessels and has antioxidant and anticancerogenic properties. in the case of polytrauma itmight have beneficial effects because of its antioxidant and anti-inflammatory properties as wellas antimicrobial activity. it is also well-known that rbr juice can induce undesirable side effects,e.g. flatulent stomach, nausea and other unpleasant reactions. therefore, it seems prospective todevelop red beetroot juice based on its natural compound composition free of undesirable side effects,which could then be used in combination with bone marrow multipotent mesenchymalstromal cells (bm mmsc) transplantation in the case of polytrauma. the aim of the study was toevaluate the therapeutic effect of allogeneic bm mmsc transplantation in rats with experimentalpolytrauma and to analyse red beetroot fractions separated on the basis of molecular weight inregard to their ingestion impact on cell transplantation efficacy. red beet juice was fractionatedby ultrafiltration (cut-off-point 20 kda). total phenolic compound concentration in the final productpractically did not decrease. the product was tested in vitro and in vivo. unlike native juice, fractionatedrbj in vitro suppressed bm mmsc adipogenic (60–71%, p < 0.05) and stimulatedosteogenic differentiation (124%, p < 0.05). experimental polytrauma in rats was modelled bycausing three fractures and haemorrhagic shock. animals were randomised in five groups: 1) normalcontrol; 2) polytrauma; 3) polytrauma + i/v bm mmsc transplantation 36 h and 5 days aftersurgery; 4) polytrauma + fractionated rbj administration per os 1ml/d, and 5) polytrauma + bmmmscs + fractionated rbj. transplantation of allogeneic bm mmscs in rats with experimentalpolytrauma stimulated bone fracture reparation, but caused plethora in viscera and dystrophicchanges in lungs. combination of bm mmscs and fractionated rbj resulted in better bone reparationand significant hematopoiesis stimulation.