Evaluation of the irritating influence of carane derivatives and their antioxidant properties in a deoxyribose degradation test

Previous studies of the propranolol monoterpene derivative (-)-4-[2-hydroxy-3-(n-isopropylamino)-propoxyimino]-cis-carane hydrochloride (kp-23) and its diastereoisomers,kp-23r and kp-23s,demonstrated different effects on the cyclic amp generating system as well as anti-inflammatory,analgesic,antihistaminic and antioxidant activity. the present study examined the influence of kp-23 and its diastereoisomers kp-23r and kp-23s on the skin-irritating activity and the mucous membrane-irritating activity as well as their influence on a late-type contact allergy in the in vivo tests. the hydroxyl radical scavenging potential of the three analogues was evaluated using their ability to inhibit fe(ii)/h2o2-induced oxidative degradation of 2-deoxyribose (2-dr) in the in vitro tests. the results obtained indicated that the hydroxyamine carane derivative did not evoke irritative changes and did not induce a late-type contact allergy in the guinea-pig. diastereoisomers of kp-23 exhibit antioxidant properties in a dose-dependent manner and protected against oh-radicals generated from the fenton reaction. copyright © 2011 by institute of pharmacology polish academy of sciences.