Effect of 4-methylpyrazole on antioxidant enzyme status and lipid peroxidation in the liver of rats after exposure to ethylene glycol and ethyl alcohol

Background: the aim of the conducted studies was to evaluate the effect of 4-methylpyrazole,increasingly used in detoxifying treatments after ethylene glycol poisoning,on the activity of some antioxidant enzymes and lipid peroxidation formation in the liver of rats after experimental co-exposure to ethylene glycol and ethyl alcohol. methods: the trials were conducted on adult male wistar rats. ethylene glycol (eg) at the dose of 3.83 g/kg bw and ethyl alcohol (ea) at the dose of 1 g/kg bw were administered po,and 4-methylpyrazole (4-mp) at the dose of 0.01 g/kg bw was administered ip. parameters of antioxidant balance were evaluated in hepatic cytosol,including the activity of the following enzymes: glutathione s-transferase (gst),glutathione reductase (gr),glutathione peroxidase (gpx) and lipid peroxidation level (tbars). results: the results suggest that evaluation of the effects of administrated 4-mp after co-exposure to eg and ea in the liver revealed statistically significant changes on antioxidant enzyme system and malondialdehyde formation. conclusion: the changes in biomarkers activity indicate a greater production of free radicals which exceeds the capability of antioxidant system,appearing with oxidative stress in the group of animals treated by 4-mp combined with eg and ea. copyright © 2012 by institute of pharmacology polish academy of sciences.