Restoration of immune system function is accelerated in immunocompromised mice by the B-cell-tropic isoxazole R-11

Background: restoration of impaired immune response in immunocompromised patients is a crucial problem. in this study we evaluated the efficacy of isoxazole r-11 in reconstitution of the immune response in immunosuppressed mice. methods: mice were given a sublethal dose (250 mg/kg b.w.) of cyclophosphamide (cp). the cellular immune response to ovalbumin (ova) and the humoral immune response to sheep erythrocytes (srbc) were generated. r-11 was administered at repetitive,intraperitoneal doses (20 μg/mouse) until determination of the immune responses: 7 and 15 doses on alternate days for cellular and humoral immune response,respectively. for phenotypic studies r-11 was given per os,at a single dose of 20 μg/mouse. the ability of r-11 to affect interleukin- 6 (il-6) production was determined in the whole human blood cell culture. results: r-11 increased the content of cd19+ cells in the spleens and lymph nodes with a concomitant decrease of cd3+ and cd4+ cells. the compound significantly accelerated restoration of both cellular and humoral immune responses,elevated the numbers of circulating leukocytes and splenocytes and normalized the blood cell picture. supplementary experiments showed that r-11 was not toxic with regard to human peripheral blood mononuclear cells (pbmc) and that it upregulated il-6 production in blood cell culture stimulated with lipopolysaccharide (lps). conclusions: we demonstrated that r-11 is likely a b-cell tropic agent which can restore both cellular and humoral immune responses in immunocompromised mice and may have a potential to be applied in therapy of immunocompromised patients. copyright © 2012 by institute of pharmacology polish academy of sciences.