Interleukin-13-induced activation of signal transducer and activator of transcription 6 is mediated by an activation of Janus kinase 1 in cultured human bronchial smooth muscle cells

Background: the current study was carried out to identify the jak molecule(s) that is involved in the il-13-induced activation of stat6 in cultured human bronchial smooth muscle cells (hbsmcs). methods: cultured hbsmcs were stimulated with il-13 in the absence and presence of jak inhibitor-i (a nonspecific jaks inhibitor),tyrphostin-ag490 (a specific jak2 inhibitor),whi-p131 (a specific jak3 inhibitor),or tyrphostin-ag9 (a specific tyk2 inhibitor),and levels of phosphorylated stat6 were measured by immunoblot analyses. results: the il-13-induced phosphorylation of stat6 was abolished by jak inhibitor-i,whereas the other inhibitors had no significant effect. conclusion: these findings indicate that the stat6 phosphorylation/activation induced by il-13 is mediated by an activation of jak1 in cultured hbsmcs. copyright © 2012 by institute of pharmacology polish academy of sciences.