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Apoptotic gene expression under influence of fludarabine and cladribine in chronic lymphocytic leukemia-microarray study
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نویسنده
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franiak-pietryga i. ,sałagacka a. ,maciejewski h. ,błoński j.z. ,borowiec m. ,mirowski m. ,robak t. ,korycka-wołowiec a.
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منبع
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pharmacological reports - 2012 - دوره : 64 - شماره : 2 - صفحه:412 -420
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چکیده
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Background: a deep insight into gene expression profiling (gep) is a key to understanding the background of disease. it can lead to identification of diagnostic and prognostic factors and then to a selection of the most appropriate therapy. the aim of this study was to evaluate differences in apoptotic gene expression in chronic lymphocytic leukemia (cll) cells influenced by fludarabine (fa) or cladribine (2-cda). methods: gep was performed in cells obtained from 10 untreated cll patients and cultured in vitro with faor 2-cda. ninety-three selected apoptotic genes were analyzed using 384 taqman® low density arrays in pooled rna. results: relevant results were found in a set of 27 genes,however,the most striking differences between fa and 2-cda were observed in the following 5 genes: bad,tnfrsf21,dapk1,card 6 and card 9. conclusion: we have found some differences in apoptotic gene expression between fa and 2-cda. these findings give prominence to genes qualifying for further studies currently conducted in our department. copyright © 2012 by institute of pharmacology polish academy of sciences.
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کلیدواژه
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Chronic lymphocytic leukemia; Cladribine; Fludarabine; Gene expression profiling; Microarrays
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آدرس
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department of hematology,medical university of lodz,ciołkowskiego 2,pl 93-510 łódź,poland,copernicus memorial hospital,ciołkowskiego 2,pl 93-510 łódź, Poland, department of pharmaceutical biochemistry,laboratory of molecular biology and pharmacogenomics,medical university of lodz,muszyńskiego 1,pl 90-151 łódź, Poland, institute of computer engineering,control and robotics i-6,wroclaw university of technology,wybrzeze wyspiańskiego 27,pl 50-320 wrocław, Poland, department of hematology,medical university of lodz,ciołkowskiego 2,pl 93-510 łódź,poland,copernicus memorial hospital,ciołkowskiego 2,pl 93-510 łódź, Poland, department of pediatrics,laboratory of immunopathology and genetics,medical university of lodz,sporna 36/60,pl 91-738 łódź, Poland, department of pharmaceutical biochemistry,laboratory of molecular biology and pharmacogenomics,medical university of lodz,muszyńskiego 1,pl 90-151 łódź, Poland, department of hematology,medical university of lodz,ciołkowskiego 2,pl 93-510 łódź,poland,copernicus memorial hospital,ciołkowskiego 2,pl 93-510 łódź, Poland, department of hematology,medical university of lodz,ciołkowskiego 2,pl 93-510 łódź,poland,copernicus memorial hospital,ciołkowskiego 2,pl 93-510 łódź, Poland
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Authors
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