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Kynurenic acid enhances expression of p21 Waf1/Cip1 in colon cancer HT-29 cells
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نویسنده
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walczak k. ,turski w.a. ,rzeski w.
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منبع
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pharmacological reports - 2012 - دوره : 64 - شماره : 3 - صفحه:745 -750
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چکیده
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Background: kynurenic acid (kyna),a tryptophan metabolite,was found in the mucus of rat small intestine. however,its role in the gastrointestinal tract is still not fully elucidated. methods: to verify whether kyna affects cell cycle regulators,the protein expression of cyclin-dependent kinase inhibitor p21 waf1/cip1 was investigated in colon adenocarcinoma ht-29 cells exposed to kyna. mtt,brdu assay and sirna technology were used to evaluate the effect of kyna on cancer cell proliferation. results: kyna significantly enhanced the expression of p21 waf1/cip1. importantly,the overexpression of this protein was involved in inhibition of proliferation and dna synthesis in ht-29 cells. conclusions: kyna may be considered as a potential chemoprevention agent against colon cancer. copyright © 2012 by institute of pharmacology polish academy of sciences.
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کلیدواژه
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Colon cancer; Kynurenic acid; p21 Waf1/Cip1; Proliferation
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آدرس
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department of medical biology,institute of rural health,jaczewskiego 2,pl 20-950 lublin,poland,department of pharmacology,medical university,chodźki 4a,pl 20-093 lublin, Poland, department of toxicology,institute of rural health,jaczewskiego 2,pl 20-950 lublin,poland,department of experimental and clinical pharmacology,medical university,jaczewskiego 8,pl 20-090 lublin, Poland, department of medical biology,institute of rural health,jaczewskiego 2,pl 20-950 lublin,poland,department of virology and immunology,institute of microbiology and biotechnology,maria-curie-skłodowska university,akademicka 19,pl 20-033 lublin, Poland
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Authors
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