Granulocyte colony-stimulating factor improves early remodeling in isoproterenol-induced cardiac injury in rats

Background: granulocyte colony-stimulating factor (g-csf) has been used in some animal models and humans with well-established cardiovascular diseases. however,its effects in the initial stage of progressive non-ischemic heart failure are unknown. methods: wistar rats (260-300 g) were divided into three groups: control (without any intervention),iso (150 mg/kg isoproterenol hydrochloride sc,once a day for two consecutive days),and iso-gcsf (50 μg/kg/d g-csf for 7 days beginning 24 h after the last administration of iso). echocardiography was performed at baseline and after 30 days of follow-up. subsequently,animals were anesthetized for hemodynamic analysis. the left ventricle was removed for analysis of interstitial collagen deposition and cardiomyocyte hypertrophy. results: isoproterenol led to left ventricular dilation (control,7.7 ± 0.14 mm; iso,8.7 ± 0.16 mm; iso-gcsf 7.8 ± 0.09 mm; p < 0.05),myocardial fibrosis (control,2.0 ± 0.18%; iso,9.1 ± 0.81%; iso-gcsf 5.9 ± 0.58%; p < 0.05) and cardiomyocyte hypertrophy (control,303 ± 10 μm2; iso,356 ± 18 μm2; iso-gcsf 338 ± 11 μm2; p < 0.05). however,g-csf partially prevented collagen deposition and left ventricular enlargement,with a slight effect on hypertrophy. characterizing a compensated stage of disease,hemodynamic analysis did not change. conclusion: g-csf administered for 7 days was effective in preventing the onset of ventricular remodeling induced by high-dose isoproterenol with decreased collagen deposition and chamber preservation. copyright © 2012 by institute of pharmacology polish academy of sciences.