Influence of 825 C>T polymorphism of G protein β3 subunit gene (GNB3) on hemodynamic response during dobutamine stress echocardiography

There is a wide interpersonal difference to dobutamine response during dobutamine stress echocardiography (dse). the aim of this study was to determine an association between gnb3 825c>t gene polymorphism,encoding the β3-subunit of g protein,and heart rate (hr),systolic (sbp) and diastolic blood pressure (dbp) response to dobutamine during dse. the study involved 119 patients with clinical indications for dse. genotyping of gnb3 825c>t (rs5443) polymorphism was based on pcr-rflp method with bsedi restriction enzyme. significantly higher levels of both resting sbp and dbp in 825t allele carriers vs. 825cc patients were revealed. hr of 825cc vs. ct + tt subjects was slower along the test period reaching marked difference at dobutamine level 30 μg/kg/min (109 ± 20 vs. 116 ± 18 bpm,respectively,p = 0.047). sbpand dbpwere markedly lower in 825cc homozygotes compared to 825t allele carriers throughout dse. it can be concluded that gnb3 825c>t polymorphism is associated with resting sbp and dbp in polish caucasian patients subjected for diagnostic dse. the polymorphism also modulate hr,sbp and dbp response during dse. copyright © 2012 by institute of pharmacology polish academy of sciences.