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Metabolic effects of the hiv protease inhibitor - saquinavir in differentiating human preadipocytes
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نویسنده
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bociaga-jasik m. ,polus a. ,góralska j. ,czech u. ,gruca a. ,oeliwa a. ,garlicki a. ,mach t. ,dembiñska-kiec a.
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منبع
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pharmacological reports - 2013 - دوره : 65 - شماره : 4 - صفحه:937 -950
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چکیده
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Background: the iatrogenic,hiv-related lipodystrophy is associated with development of the significant metabolic and cardiovascular complications. the underlying mechanisms of antiretroviral (arv) drugs are not completely explored. methods: the aim of the study was to characterize effects of the protease inhibitor (pi) - saquinavir (sqv) on metabolic functions,and gene expression during differentiation in cells (chub-s7) culture. results: sqv in concentrations observed during antiretroviral therapy (art) significantly decreased mitochondrial membrane potential (mmp),oxygen consumption and atpgeneration. the effects were greater in already differentiated cells. this was accompanied by characteristic changes in the expression of the genes involved in endoplasmic reticulum (er) stress,and differentiation (lipid droplet formation) process such as: wnt10a,c/ebp?,aft4,cidec,adipoq,lpin1. conclusions: the results indicate that sqv affects not only metabolic (mitochondrial) activity of adipocytes,but affects the expression of genes related to differentiation and to a lesser extent to cell apoptosis.
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کلیدواژه
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Adipocyte; Apoptosis; Gene expression; Hiv-Associated lipodystrophy; Mitochondrial toxicity; Oxidative stress; Protease inhibitors
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آدرس
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hepatology and infectious diseases,department of infectious diseases,jagiellonian university,oeniadeckich 5,pl 31-501 krakow, Poland, department of clinical biochemistry,jagiellonian university,collegium medicum,kopernika 15a,pl 31-501 krakow, Poland, department of clinical biochemistry,jagiellonian university,collegium medicum,kopernika 15a,pl 31-501 krakow, Poland, department of clinical biochemistry,jagiellonian university,collegium medicum,kopernika 15a,pl 31-501 krakow, Poland, department of clinical biochemistry,jagiellonian university,collegium medicum,kopernika 15a,pl 31-501 krakow, Poland, department of clinical biochemistry,jagiellonian university,collegium medicum,kopernika 15a,pl 31-501 krakow, Poland, hepatology and infectious diseases,department of infectious diseases,jagiellonian university,oeniadeckich 5,pl 31-501 krakow, Poland, hepatology and infectious diseases,department of infectious diseases,jagiellonian university,oeniadeckich 5,pl 31-501 krakow, Poland, department of clinical biochemistry,jagiellonian university,collegium medicum,kopernika 15a,pl 31-501 krakow, Poland
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Authors
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