Investigation into the role of Cu/Zn-SOD delivery system on its antioxidant and antiinflammatory activity in rat model of peritonitis

Background the current study evaluated the role of delivery system (solution,conventional liposomes and peg-ylated liposomes) on superoxide dismutase (sod) antioxidant and antiinflammatory properties in a rat model of lipopolysaccharide (lps)-induced peritonitis. methods fifty male albino rats (wistar-bratislava) were divided into five groups (n = 10). control group received saline and the other four groups received intraperitoneal injections of lps (5 mg/kg). among the lps-injected groups,one was lps control group and the other three groups received the endotoxin injection 30 min after receiving the same dose of sod (500 u/kg,ip) in different delivery systems: saline solution (sod-s),conventional liposomes (sod-l) or peg-ylated liposomes (sod-pl). the animals were euthanized 6 h after lps injection,blood samples were collected and acute phase response (total and differential leukocytes count; tumor necrosis factor α),antioxidants (total antioxidants; reduced glutathione),oxidative stress (total oxidants; lipid peroxidation) and nitrosative stress (nitric oxide metabolites; nitrotyrosine) were evaluated. results intraperitoneal administration of lps to rats induced a marked inflammatory and oxidative response in plasma. on the other hand,all sod formulations had protective effect against endotoxin-induced inflammation and oxidative/nitrosative stress,but peg-ylated liposomes had the most significant activity. thus,sod-pl administration significantly reduced the effects of lps on bone marrow acute phase response,the oxidative status and production of nitric oxide metabolites,while increasing the markers of antioxidant response in a significant manner. conclusion sod supplementation interferes both with inflammatory and oxidative pathways involved in lps-induced acute inflammation,peg-ylated liposomal formulation being of choice among the tested delivery systems. © 2014 institute of pharmacology,polish academy of sciences.