Development of an alternative non-obese non-genetic rat model of type 2 diabetes using caffeine and streptozotocin

Background the aim of the present study was to develop an alternative non-obese non-genetic rat model of type 2 diabetes (t2d). methods six-week-old male sd rats were randomly divided into six groups,namely: normal control (nc),diabetic control (dbc),caffeine 5 mg/kg bw + stz (caf5),caffeine 10 mg/kg bw + stz (caf10),caffeine 20 mg/kg bw + stz (caf20) and caffeine 40 mg/kg bw + stz (caf40) and were fed a normal rat pellet diet and drinking water ad libitum throughout the experimental period. after a one week acclimatization period,diabetes was induced in the animals in dbc and all caf groups with an injection (i.p.) of the respective dosages of caffeine (mg/kg bw) 15 min before the injection of stz (65 mg/kg bw) when normal saline was injected to the dbc group instead of caffeine. the nc group received normal saline and buffer instead of caffeine and stz,respectively. one week after the stz injection,animals with non-fasting blood glucose > 300 mg/dl were considered as diabetic. three weeks after the stz injection,the animals in the caf5 and caf10 groups were eliminated from the study due to the severity of diabetes and the experiment was continued with the remainder groups for a 13 weeks period. results and conclusion the data of food and fluid intake,body weight,blood glucose,glucose tolerance test,homa-ir,homa-beta,serum insulin,fructosamine,lipid profile and organ specific enzymes,anti-diabetic drug response tests,and pancreatic histopathology suggest that caf20 group can be a better alternative non-genetic model of non-obese t2d. © 2014 institute of pharmacology,polish academy of sciences.