Luteolin 8-C-β-fucopyranoside downregulates IL-6 expression by inhibiting MAPKs and the NF-κB signaling pathway in human monocytic cells

Numerous studies have been suggested that derivatives can improve the effects of original substances. therefore,we made luteolin derivative luteolin 8-c-β-fucopyranoside (lu8c-fp) for better anti-inflammatory and anti-cancer effects. in a previous study,we demonstrated that lu8c-fp inhibits invasion of human breast cancer cells via suppression of matrix metalloproteinase 9 and il-8,which play major roles in tumor progression and cancer cell invasion. various stimuli trigger inflammatory responses by inducing pro-inflammatory cytokines and chemokines in thp-1 cells. il-6 induces inflammation via inducing various cytokines and appears to be a potential mediator of inflammatory diseases. here,we investigated the precise mechanism by which lu8c-fp inhibited phorbol 12-myristate 13-acetate-induced il-6 mrna and protein expression. we showed lu8c-fp downregulated il-6 expression by inhibiting mitogen-activated protein kinases and the nuclear factor-kappab signaling pathway in human monocytic cells. furthermore,lu8c-fp exerts less cytotoxicity than luteolin and also it has specific inhibitory effect on il-6 expression. however,luteolin has a variety of inhibitory effects on pro-inflammatory cytokines and chemokines. our in vitro studies may provide valuable information leading to the use of lu8c-fp to treat inflammatory diseases caused by il-6. © 2015 institute of pharmacology,polish academy of sciences. published by elsevier urban & partner sp. z o.o. all rights reserved.