Involvement of c-Myc-mediated transient receptor potential melastatin 8 expression in oxaliplatin-induced cold allodynia in mice

Background oxaliplatin,a platinum-based chemotherapeutic agent,induces acute cold allodynia and dysesthesia. cold-sensitive transient receptor potential channels (trpm8 and trpa1) have been implicated as candidates to mediate oxaliplatin-induced cold allodynia and hyperalgesia,but precise roles of these channels remain unclear. in this study,we investigated the role of trpm8 in oxaliplatin-induced cold allodynia. methods oxaliplatin was injected intraperitoneally in mice. cold allodynia was evaluated by the acetone test. expression levels of trpm8 mrna and protein were measured using reverse transcription-polymerase chain reaction and western blotting,respectively. results oxaliplatin-induced cold allodynia was alleviated by the trpm8 blockers n-(2-aminoethyl)-n-[4-(benzyloxy)-3-methoxybenzyl]-n′-(1s)-1-(phenyl) ethyl] urea and tc-i 2014. oxaliplatin increased the expression levels of trpm8 mrna and protein in the dorsal root ganglia and plantar skin,respectively. prophylactic administration of the c-myc inhibitor 10058-f4 prevented cold allodynia and the increase of trpm8 mrna after oxaliplatin injection. conclusion these results suggest that oxaliplatin induces cold allodynia through the increase of c-myc-mediated trpm8 expression in primary sensory neurons. © 2016 institute of pharmacology,polish academy of sciences.