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Determination of Pediobius sp. (Hymenoptera: Eulophidae), A New Species Record of Endoparasitoid Associate with Beet Armyworm, Spodoptera exigua (Lepidoptera: Noctuidea) from Malaysia using DNA Barcode
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نویسنده
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Ghazali S. Z. ,Md-Zain B. M. ,Yaakop S.
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منبع
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pertanika journal of tropical agricultural science - 2014 - دوره : 37 - شماره : 2 - صفحه:285 -291
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چکیده
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Spodoptera exigua (lepidoptera: noctuidea) is a polyphagous pest that attacks many important agricultural crops. identifying an insect specimen is a crucial step in entomology. this study demonstrated a molecular method to identify the species of pest and its parasitoid in the case of a lack of a morphological identification key. to facilitate the identification of these pest-parasitoid species, a dna barcoding approach was used for accurate and time-consuming identification based on nucleotide sequencing analysis of the mitochondrial cytochrome oxidase subunit i (coi) gene. dna barcoding sequences successfully identified both pest and parasitoid species by comparing barcode sequences to the genbank database. this study provided evidence of pediobius sp. as a parasitoid of s. exigua in malaysia.
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کلیدواژه
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DNA barcoding ,COI gene ,Spodoptera exigua ,Pediobius sp. ,fern
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آدرس
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Universiti Kebangsaan Malaysia, School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Malaysia, Universiti Kebangsaan Malaysia, School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Malaysia, Universiti Kebangsaan Malaysia, School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Malaysia
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Authors
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