Micropropagation of Asam Karanda (Carissa carandas Linn)

An efficient micropropagation protocol was developed for c. carandas using nodal segment as the explants. murashige and skoog medium (ms), supplemented with 2.0 – 4.0 mg/l benzylaminopurine (ba), induced an average of 3.6 - 5.5 shoots/explants but the number of multiple shoots formed was not significantly different. however, the addition of iba combined with ba had shown a reducing trend in the number of shoot being formed. the ms medium supplemented with 2.0 mg/l 3-indolebutyric acid (iba) was found to be effective for rooting of micro-shoots. after the acclimatization process, 84% ± 4.3% of the in vitro plantlets survived but there was a reduction in the number of survived plantlets (59 ± 3.7%) after 4 weeks of incubation in the green house condition.