Apoptosis and Tumour Cell Death in Response to Pro-apoptotic Gene

The process of cell death, or apoptosis, is commonly defined by its distinct morphological characteristic. apoptosis is considered a vital component of various processes including normal cell turnover, proper development and functioning of the immune system, the cellular antiviral response pathway, embryonic development and chemical-induced cell death. in this study, retroviral vector was utilized as the gene delivery vehicles and the tumour-specific viral death effector vp3 as pro-apoptotic agent for malignant colon cancer cells treatment. here, pro-apoptotic gene inducing apoptosis in ct26 colon tumour cells is reported. in addition, the activation of a typical apoptotic programme was observed in response to this therapeutic agent. an increased number of colon tumour cells suffered apoptosis upon treatment with pro-apoptotic agent. as a result, these tumour cells displayed loss of membrane asymmetry and impermeability, cell shrinkage, nuclear condensation and dna fragmentation under h&e images. in the presence of vp3, tem also confirmed that vp3-treated ct26 tumour cells showed apoptotic features. these results suggested that vp3 was a potential pro-apoptotic agent and the apoptosis induced by vp3 was a key anti-tumour mechanism