Gender Identification of Domesticated Chicken Using a PCR-based Method

The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. thus, a pcr based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. feathers of a day up to a week old chicks were collected for the molecular analysis using the pcr targeting the chd-w and chd-z genes in the chicken’s chromosome z and chromosome w, respectively. the results of this study showed that the pcr based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method