Protein Expression of Late Elongated Hypocotyl (LHY) Homolog Genes of Teak in Escherichia coli

Expression of an isolated gene in a system that directly translates it into a protein is an important step to study the protein encoded by the gene. the isolated gene can be expressed in vivo by a heterologous system. in this study, a bacteria system was used to translate the tectono grandis late elongated hypocotyl (tg-lhy) gene, which was isolated from flowering tissues of teak (tectona grandis). the gene was cloned into the pet 14b vector (novagen) and transformed into bl 21 (de3) /plyss and rosetta 2 expression host cells (novagen). rosetta 2 host cell has been found to be a good candidate to express the tg-lhy protein from plant origin, as it recognizes the codon that was found in plant but rarely used in bacteria. the expressed protein was about an expected size , which was 90 kd. western blot analysis using antibody against his-tag, which was fused to the tg-lhy protein, proved that the expressed protein was tg-lhy protein.