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effective induction of hairy roots in persian poppy (papaver bracteatum lindl.) using sonication method
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نویسنده
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qavami n ,azizi m ,yazdian f ,qaderi a ,nemati h
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منبع
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journal of medicinal plants - 2018 - دوره : 18 - شماره : 65 - صفحه:73 -82
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چکیده
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Background: papaver bracteatum lindl. commonly known as iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids.objective: to evaluate the hairy root culture as a novel method for thebaine production.methods: to optimize the hairy root culture of p. bracteatum, five strains of agrobacterium rhizogenes (atcc15834, c318, a13, 9453 and a4) were used by means of sonicationassisted agrobacteriummediated transformation (saat).results: strain c318 proved to be more efficient than the other strains to hairy root induction ability. among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41 deg;c for 5 min. transgenic status of the hairy roots was confirmed by pcr using rolb specific primers.conclusion: strain c318 proved to be more efficient than the other strains to hairy root induction ability. among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41 deg;c for 5 min. transgenic status of the hairy roots was confirmed by pcr using rolb specific primers.
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کلیدواژه
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papaver bracteatum lindl. hairy root ,sonication-assisted agrobacterium-mediated transformation ,thebaine ,thebeine
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آدرس
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ferdowsi university of mashhad, faculty of agriculture, department of horticultural sciences, ایران, ferdowsi university of mashhad, faculty of agriculture, department of horticultural sciences, ایران, university of tehran, faculty of new sciences and technologies, department of life science engineering, ایران, academic center for education, culture and research (acecr), institute of medicinal plants, medicinal plants research center, ایران, ferdowsi university of mashhad, faculty of agriculture, department of horticultural sciences, ایران
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Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method
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Authors
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Qavami N ,Azizi M ,Yazdian F ,Qaderi A ,Nemati H
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Abstract
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Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids.Objective: To evaluate the hairy root culture as a novel method for thebaine production.Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of SonicationAssisted Agrobacteriummediated Transformation (SAAT).Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41 deg;C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41 deg;C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
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Keywords
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Papaver bracteatum Lindl. Hairy root ,Sonication-assisted Agrobacterium-mediated transformation ,Thebaine ,Thebeine
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