Classification of Iranian Garlic (Allium sativum L.) Ecotypes Using RAPD Marker

Garlic is a valuable medicinal plant with variability in desirablemorphological and physiological characteristics. the analysis of genetic diversity plays animportant role in breeding programs. the rapd technique could be very effective indetecting genetic variation in garlic.objective: the objective of the present work was to detect molecular polymorphismamong iranian garlic ecotypes by rapd technique.methods: random amplified polymorphic dna (rapd) marker utilized to assess thegenetic diversity among twenty-two different ecotypes of the iranian garlic. genomicdna was extracted from young leaves using dellaporta method. rapd amplification wasperformed using ten 10-mers arbitrary primers.results: results indicated that five out of ten pair primers had no amplification. a totalof 35 rapd bands were produced, 31 of which (88.5%) were polymorphic. the similaritymatrices and dendrogram were obtained using upgma algorithms.conclusion: in conclusion the entire population was grouped into four clusters with 3,9, 1 and 9 ecotypes. no significant relationship between genetic diversity detected byrapd technique and geographical origins.