synthesis, characterization, anti-microbial activity of silver nanoparticles, and molecular interaction studies of therapeutic agents of pongamia pinnata (pp) and azadirachta indica (ai)

Background: antimicrobial activity has been reported against a range of bacteria, including multidrug-resistant strains for silver nanoparticles (agnps) due to penetration of nps into the cell. agnps can be used in combination with other antibacterial agents to create a synergistic effect. objective: the present work aims at the preparation of agnps in methanol extracts of the dried bark powder of two medicinally important plants, viz., pongamiapinnata (pp) and azadirachtaindica (ai) and their therapeutic uses. method: uv-visible, ftir spectral techniques and sem images were used to characterize the nps. these bio-nps were tested for this response against mycobacteria such as streptomycin and fluconazole, viruses, bacteria, and fungi. results: antibacterial activity, antifungal activity and detailed molecular interaction between the therapeutic constituents of pp and ai against human mixtard insulin (hmi) and histamine were studied by in silico docking analysis. the antimicrobial studies of both pp and ai yielded positive results in our attempt to investigate the mycobacterial study against two human pathogenic organisms. conclusion: extensive in-silico docking study showed that the decreasing order of non-covalent interactions of four therapeutic components is isopongachromene > karanjin > quercetin > azadirachtin against insulin and isopongachromene > azadirachtin > karanjin > quercetin against histamine.