topical formulation of a skin-natural wound healing remedy based on traditional persian medicine

Background: historical documents highlight various multifocal products for wound management, including the zaroor arbaee formulation, which comprises commiphora myrrha, boswellia sp., astragalus fasciculifolius, and dracaena sp. objective: this study investigates a novel topical formulation for skin wound healing based on traditional persian medicine, emphasizing these botanicals. methods: a methanol extract was prepared, and an mtt assay was used to assess cytotoxicity on normal liver cells. antimicrobial activity was evaluated using the microdilution method according to clsi guidelines. a 5% topical cream was formulated, and total phenolic, flavonoid, and tannin contents were measured via spectrophotometry. high-performance thin-layer chromatography (hptlc) was employed for phytochemical profiling, and essential oil extraction was performed to identify volatile constituents. organoleptic and physicochemical tests were also conducted on the cream. results: the mtt assay showed no cytotoxicity, and antimicrobial tests confirmed significant antifungal activity. total phenolic, flavonoid, and tannin contents were 7.59 ± 0.31 mg, 0.54 ± 0.007 mg, and 2.07 ± 0.13 mg per 1.5 g of extract in a 30 g cream, respectively. essential oil analysis identified furanoeudesma-1,3-diene (32.84%) and curzerene (14.94%) as major constituents. additional assessments confirmed the cream’s stability and spreadability. conclusion: zaroor arbaee appears to be a promising traditional remedy for skin wound healing.