isolation and purification of apigenin, quercetin and apigenin 7-o-glycoside from apium graveolens l., petroselinum crispum (mill.) fuss, allium cepa l., respectively

Background: flavonoids, a group of natural substances, are found in flowers, fruits, roots and stems. these natural products are well known for their health-beneficial effects. hence, isolation and purifying of flavonoids from the ingredients is worthwhile. flavonoids are now considered indispensable in various nutraceutical, pharmaceutical, medicinal and cosmetic applications. apigenin, quercetin, and apigenin-7-o-glycoside have therapeutic properties that these two are in the flavonoid group. objectives: in this study, apigenin, quercetin, and apigenin 7-o-glycoside were purified by column chromatography and identified by nmr and hplc uv-visible techniques. methods: total extracts of three plants (apium graveolens, petroselinum crispum, allium cepa) were divided into different solvent fractions. column chromatography is used to purify these fractions. finally, 1h-nmr, 13c-nmr, and mass spectrometry techniques were used for identification. also, the hplc technique was used for identifying these compounds and standardized. results: in the present work, apigenin was extracted from ethyl acetate fraction of apium graveolens seeds. high purity of apigenin 7-o-glycoside isolated from the butanol fraction of petroselinum crispum aerial parts. also, ethyl acetate fraction isolated quercetin from the allium cepa. identification of the data obtained from 1h-nmr and 13c-nmr, which were proofed by comparison with the sources of purified compounds. conclusion: the methods used in this research were able to produce these three compounds with a high percentage of purity to be used in industry as standards.background: flavonoids, a group of natural substances, are found in flowers, fruits, roots and stems. these natural products are well known for their health-beneficial effects. hence, isolation and purifying of flavonoids from the ingredients is worthwhile. flavonoids are now considered indispensable in various nutraceutical, pharmaceutical, medicinal and cosmetic applications. apigenin, quercetin, and apigenin-7-o-glycoside have therapeutic properties that these two are in the flavonoid group. objectives: in this study, apigenin, quercetin, and apigenin 7-o-glycoside were purified by column chromatography and identified by nmr and hplc uv-visible techniques. methods: total extracts of three plants (apium graveolens, petroselinum crispum, allium cepa) were divided into different solvent fractions. column chromatography is used to purify these fractions. finally, 1h-nmr, 13c-nmr, and mass spectrometry techniques were used for identification. also, the hplc technique was used for identifying these compounds and standardized. results: in the present work, apigenin was extracted from ethyl acetate fraction of apium graveolens seeds. high purity of apigenin 7-o-glycoside isolated from the butanol fraction of petroselinum crispum aerial parts. also, ethyl acetate fraction isolated quercetin from the allium cepa. identification of the data obtained from 1h-nmr and 13c-nmr, which were proofed by comparison with the sources of purified compounds. conclusion: the methods used in this research were able to produce these three compounds with a high percentage of purity to be used in industry as standards.

isolation and purification of apigenin, quercetin and apigenin 7-o-glycoside from apium graveolens l., petroselinum crispum (mill.) fuss, allium cepa l., respectively

background: flavonoids, a group of natural substances, are found in flowers, fruits, roots and stems. these natural products are well known for their health-beneficial effects. hence, isolation and purifying of flavonoids from the ingredients is worthwhile. flavonoids are now considered indispensable in various nutraceutical, pharmaceutical, medicinal and cosmetic applications. apigenin, quercetin, and apigenin-7-o-glycoside have therapeutic properties that these two are in the flavonoid group. objectives: in this study, apigenin, quercetin, and apigenin 7-o-glycoside were purified by column chromatography and identified by nmr and hplc uv-visible techniques. methods: total extracts of three plants (apium graveolens, petroselinum crispum, allium cepa) were divided into different solvent fractions. column chromatography is used to purify these fractions. finally, 1h-nmr, 13c-nmr, and mass spectrometry techniques were used for identification. also, the hplc technique was used for identifying these compounds and standardized. results: in the present work, apigenin was extracted from ethyl acetate fraction of apium graveolens seeds. high purity of apigenin 7-o-glycoside isolated from the butanol fraction of petroselinum crispum aerial parts. also, ethyl acetate fraction isolated quercetin from the allium cepa. identification of the data obtained from 1h-nmr and 13c-nmr, which were proofed by comparison with the sources of purified compounds. conclusion: the methods used in this research were able to produce these three compounds with a high percentage of purity to be used in industry as standards.background: flavonoids, a group of natural substances, are found in flowers, fruits, roots and stems. these natural products are well known for their health-beneficial effects. hence, isolation and purifying of flavonoids from the ingredients is worthwhile. flavonoids are now considered indispensable in various nutraceutical, pharmaceutical, medicinal and cosmetic applications. apigenin, quercetin, and apigenin-7-o-glycoside have therapeutic properties that these two are in the flavonoid group. objectives: in this study, apigenin, quercetin, and apigenin 7-o-glycoside were purified by column chromatography and identified by nmr and hplc uv-visible techniques. methods: total extracts of three plants (apium graveolens, petroselinum crispum, allium cepa) were divided into different solvent fractions. column chromatography is used to purify these fractions. finally, 1h-nmr, 13c-nmr, and mass spectrometry techniques were used for identification. also, the hplc technique was used for identifying these compounds and standardized. results: in the present work, apigenin was extracted from ethyl acetate fraction of apium graveolens seeds. high purity of apigenin 7-o-glycoside isolated from the butanol fraction of petroselinum crispum aerial parts. also, ethyl acetate fraction isolated quercetin from the allium cepa. identification of the data obtained from 1h-nmr and 13c-nmr, which were proofed by comparison with the sources of purified compounds. conclusion: the methods used in this research were able to produce these three compounds with a high percentage of purity to be used in industry as standards.