identification of isoflavonoids in antioxidant effective fraction of arum rupicola boiss. leaves

Background: arum rupicola boiss. (araceae family) is used by the native people of southern areas of iran as a soup called kardeh soup . several flavonoids and phenol compounds have been identified from arum species. objective: the aim of this study was to evaluate antioxidant effect and total phenol contents as well as cytotoxic activity of the leaves of a. rupicola. methods: antioxidant activity of total methanol extract and fractions including nhexane, chloroform, ethyl acetate and water residue were evaluated using frap and dpph methods. total phenol content was measured using folinciocalteu method. cytotoxic activity of the extract and fractions were investigated against human breast cancer mcf-7, mda-mb-231, and t47d cell lines by mtt assay. further phytochemical isolation was done on the water residue using column chromatography. results: according to the results, water residue showed the lowest ic50 value (186.7 μg/ml) and the total methanol extract showed the most antioxidant power (163.62 mmol feso4/100 g extract) and phenol content (135 μmol gallic acid/g extract). the hexane fraction also showed the highest cytotoxic effect against mcf-7 breast cancer cell line with ic50 equal to 118.9 μg/ml. phytochemical analysis of the water residue resulted in isolation and identification of three isoflavonoids named orobol, genistein and genistein 8-c-glucoside. conclusion: based on the identification of isoflavonoid compounds in this plant, its ability to be used as a phytoestrogenic supplement can be considered in future studies.

Identification of isoflavonoids in antioxidant effective fraction of Arum rupicola Boiss. leaves

Background: Arum rupicola Boiss. (Araceae Family) is used by the native people of southern areas of Iran as a soup called kardeh soup . Several flavonoids and phenol compounds have been identified from Arum species. Objective: The aim of this study was to evaluate antioxidant effect and total phenol contents as well as cytotoxic activity of the leaves of A. rupicola. Methods: Antioxidant activity of total methanol extract and fractions including nhexane, chloroform, ethyl acetate and water residue were evaluated using FRAP and DPPH methods. Total phenol content was measured using FolinCiocalteu method. Cytotoxic activity of the extract and fractions were investigated against human breast cancer MCF7, MDAMB231, and T47D cell lines by MTT assay. Further phytochemical isolation was done on the water residue using column chromatography. Results: According to the results, water residue showed the lowest IC50 value (186.7 micro;g/ml) and the total methanol extract showed the most antioxidant power (163.62 mmol FeSO4/100 g extract) and phenol content (135 micro;mol Gallic acid/g extract). The hexane fraction also showed the highest cytotoxic effect against MCF7 breast cancer cell line with IC50 equal to 118.9 mu;g/ml. Phytochemical analysis of the water residue resulted in isolation and identification of three isoflavonoids named orobol, genistein and genistein 8cglucoside. Conclusion: Based on the identification of isoflavonoid compounds in this plant, its ability to be used as a phytoestrogenic supplement can be considered in future studies.