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cytotoxic activity of centaurea albonitens turrill aerial parts in colon and breast cancer cell lines
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نویسنده
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esmaeili somayeh ,asadi mahya ,mosaddegh mahmoud ,mohammadi motamed saeed ,hamzeloo-moghadam maryam
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منبع
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journal of medicinal plants - 2021 - دوره : 20 - شماره : 78 - صفحه:59 -67
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چکیده
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Background: cancer is the second cause of death in developed countries. colon and breast cancers are among the most prevalent ones. research focusing on finding new natural products with fewer side effects to fight cancer is increasing. objectives: the present study aimed to evaluate the cytotoxicity of centaurea albonitens turrill methanol extract and its fractions against colon and breast cancer cell lines and a normal cell line of bovine kidney cells. methods: the methanol extract and petroleum ether, chloroform and aqueous fractions were provided from the aerial parts of c. albonitens by maceration method in three days. each day the solvent was refreshed. colon (ht29) and breast (mcf7) cell lines were treated with the extract/fractions for 48 h for evaluating the cytotoxic activity by mtt assay. the apoptotic induction potential was also evaluated with the hoechst 33258 staining method. results: the most considerable effect was reported from the chloroform fraction with ic50 values of 25.6 and 25.1 mu;g/ml in mcf7 and ht29 cells, respectively. in hoechst staining, condensed chromatin of the apoptotic cells was observed in both cell lines. conclusion: centaurea albonites can be suggested for further cancer research studies.
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کلیدواژه
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centaurea albonitens ,cytotoxicity ,mtt assay ,hoechst 33258 ,mcf-7 ,hoechst 33258 ,mcf-7
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آدرس
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shahid beheshti university of medical sciences, school of traditional medicine, traditional medicine and materia medica research center, department of traditional pharmacy, iran, islamic azad university tehran medical sciences, faculty of pharmacy and pharmaceutical sciences, department of pharmacognosy, iran, shahid beheshti university of medical sciences, school of pharmacy, departtment of pharmacognosy, iran, islamic azad university tehran medical sciences, faculty of pharmacy and pharmaceutical sciences, department of pharmacognosy, iran, shahid beheshti university of medical sciences, school of traditional medicine, traditional medicine and materia medica research center, department of traditional pharmacy, iran
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پست الکترونیکی
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mhmoghadam@sbmu.ac.ir
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Cytotoxic activity of Centaurea albonitens Turrill aerial parts in colon and breast cancer cell lines
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Authors
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Esmaeili Somayeh ,Asadi Mahya ,Mosaddegh Mahmoud ,Mohammadi Motamed Saeed ,Hamzeloo-Moghadam Maryam
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Abstract
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Background: Cancer is the second cause of death in developed countries. Colon and breast cancers are among the most prevalent ones. Research focusing on finding new natural products with fewer side effects to fight cancer is increasing. Objectives: The present study aimed to evaluate the cytotoxicity of Centaurea albonitens Turrill methanol extract and its fractions against colon and breast cancer cell lines and a normal cell line of bovine kidney cells. Methods: The methanol extract and petroleum ether, chloroform and aqueous fractions were provided from the aerial parts of C. albonitens by maceration method in three days. Each day the solvent was refreshed. Colon (HT29) and breast (MCF7) cell lines were treated with the extract/fractions for 48 h for evaluating the cytotoxic activity by MTT assay. The apoptotic induction potential was also evaluated with the Hoechst 33258 staining method. Results: The most considerable effect was reported from the chloroform fraction with IC50 values of 25.6 and 25.1 mu;g/mL in MCF7 and HT29 cells, respectively. In Hoechst staining, condensed chromatin of the apoptotic cells was observed in both cell lines. Conclusion: Centaurea albonites can be suggested for further cancer research studies.
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Keywords
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Centaurea albonitens ,Cytotoxicity ,MTT assay ,Hoechst 33258 ,MCF-7 ,Hoechst 33258 ,MCF-7
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