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regulation effects of trigonella foenum-graecum seed extract on a mouse model of allergic asthma
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نویسنده
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athari shamsadin ,keshavarz mansoor ,khodadoost mahmood ,hamzeloo-moghadam maryam ,abbassian alireza ,choopani rasool
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منبع
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journal of medicinal plants - 2021 - دوره : 20 - شماره : 78 - صفحه:26 -35
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چکیده
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Background: trigonella foenumgraecum l. (fenugreek) seeds have a long history of treating allergic asthma known as ldquo;rabv rdquo; in persian medicine. the protective effects of fenugreek on asthma have been noted in persian medicine texts. objective: the aim of the present study was to investigate the protective effects of aqueous fenugreek seed extract (afse) on the reduction of th2 cytokines via decreasing the levels of mrna expression of th2 cytokines in bronchoalveolar lavage fluid (balf). methods: 28 female balb/c mice were divided into four groups of seven animals. negative and positive control groups received phosphatebuffered saline and ovalbumin (ova), respectively. the remaining two groups were firstly sensitized with ova to induce asthma and then received theophylline and afse. thereafter, the mrna expressions of th2type interleukins (il4, il5, and il13) and mucin5 as well as the concentrations of il5, il13, and il33 in balf samples were measured and pathological alterations of lung tissues were analyzed. results: afse treatment of balb/c mice significantly decreased the number of eosinophils, the mrna expressions of il4, il5, il13, and mucin5 and the concentrations of il5, il13, and il33 in their balf specimens. it also considerably prevented peribronchial and perivascular inflammations, mucus hypersecretion, and goblet cell hyperplasia in lung tissues in comparison to ovasensitized mice. conclusion: the present study demonstrated that the aqueous fenugreek seeds extract could be an alternative medication for the treatment of allergic asthma.
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کلیدواژه
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asthma ,trigonella foenum-graecum ,allergy ,persian medicine ,type 2 cytokines
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آدرس
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zanjan university of medical sciences, school of medicine, department of immunology, iran, tehran university of medical sciences, school of medicine, department of physiology, iran, shahid beheshti university of medical sciences, school of traditional medicine, department of traditional medicine, iran, shahid beheshti university of medical sciences, school of traditional medicine, traditional medicine and materia medica research center, department of traditional pharmacy, iran, tehran university of medical sciences, school of traditional medicine, department of iranian traditional medicine, iran, shahid beheshti university of medical sciences, school of traditional medicine, traditional medicine and materia medica research center, department of traditional medicine, iran
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پست الکترونیکی
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rchoopani@sbmu.ac.ir
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Regulation effects of Trigonella foenum-graecum seed extract on a mouse model of allergic asthma
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Authors
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Athari Shamsadin ,Keshavarz Mansoor ,Khodadoost Mahmood ,Hamzeloo-Moghadam Maryam ,Abbassian Alireza ,Choopani Rasool
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Abstract
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Background: Trigonella foenumgraecum L. (fenugreek) seeds have a long history of treating allergic asthma known as ldquo;Rabv rdquo; in Persian medicine. The protective effects of fenugreek on asthma have been noted in Persian medicine texts. Objective: The aim of the present study was to investigate the protective effects of aqueous fenugreek seed extract (AFSE) on the reduction of Th2 cytokines via decreasing the levels of mRNA expression of Th2 cytokines in bronchoalveolar lavage fluid (BALF). Methods: 28 female Balb/c mice were divided into four groups of seven animals. Negative and positive control groups received Phosphatebuffered saline and ovalbumin (OVA), respectively. The remaining two groups were firstly sensitized with OVA to induce asthma and then received Theophylline and AFSE. Thereafter, the mRNA expressions of Th2type interleukins (IL4, IL5, and IL13) and mucin5 as well as the concentrations of IL5, IL13, and IL33 in BALF samples were measured and pathological alterations of lung tissues were analyzed. Results: AFSE treatment of Balb/c mice significantly decreased the number of eosinophils, the mRNA expressions of IL4, IL5, IL13, and mucin5 and the concentrations of IL5, IL13, and IL33 in their BALF specimens. It also considerably prevented peribronchial and perivascular inflammations, mucus hypersecretion, and goblet cell hyperplasia in lung tissues in comparison to OVAsensitized mice. Conclusion: The present study demonstrated that the aqueous fenugreek seeds extract could be an alternative medication for the treatment of allergic asthma.
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Keywords
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Asthma ,Trigonella foenum-graecum ,Allergy ,Persian medicine ,Type 2 Cytokines
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