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quality control of rosa × damascena flowers from layzangan of fars province in iran
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نویسنده
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mosleh ghazaleh ,badr parmis ,azadi amir ,afsari sardari forough ,iraji aida ,khademian sedigheh ,abolhassanzadeh zohreh ,mohagheghzadeh abdolali
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منبع
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journal of medicinal plants - 2021 - دوره : 20 - شماره : 78 - صفحه:48 -58
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چکیده
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Background: damask rose (dr) with the scientific name of rosa × damascena herrm. is a popular medicinal plant belonging to the rosaceae family. according to the literature, dr cultivation was originally developed in the layzangan valley, darab region of fars province in iran. objective: in this study, quality control and standardization of layazangan dr flowers were investigated. methods: histological, physicochemical, and heavy metal analysis as well as the chromatographic fingerprints including gcms and hplc analysis were studied. results: according to the microscopic observations, secretory and simple trichromes in sepal, rosette crystals in ovule, and the tricolporate shape of pollen grain were found to be the major characteristics in dr flowers. the results of heavy metal analysis revealed that the amounts of zinc (82.5 ppm), copper (4.02 ppm), lead (0.11 ppm), and cadmium (not detected) were less than maximum permitted amounts. the essential oil of fresh petals was obtained by hydrodistillation and was analyzed by gcms method. twentythree components were identified representing 98.66 % of the total essential oil composition. citronellol (41.44 %), nonadecane (16.44 %), and heneicosane (10.58 %) were the main components. according to hplc analysis results, quercetin was determined as 7300.5 mu;g/g dried dr petals. conclusion: the essential oil obtained from layzangan dr is rich in citronellol compound. on the other hand, the diagnostic characteristics presented in this study can help in better quality control and standardization of dr samples. it could also provide information for the authentication of relevant undeclared samples as well as the detection of adulterated materials.
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کلیدواژه
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damask rose ,histological analysis ,gc-ms ,hplc
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آدرس
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shiraz university of medical sciences, phytopharmaceutical technology and traditional medicine incubator, iran, shiraz university of medical sciences, phytopharmaceutical technology and traditional medicine incubator, pharmaceutical sciences research center, iran, shiraz university of medical sciences, faculty of pharmacy, department of pharmaceutics, iran, shiraz university of medical sciences, faculty of pharmacy, department of phytopharmaceuticals (traditional pharmacy), iran, shiraz university of medical sciences, medicinal and natural products chemistry research center, iran, shiraz university of medical sciences, faculty of pharmacy, department of phytopharmaceuticals (traditional pharmacy), iran, shahrekord university of medical sciences, medical plants research center, iran, shiraz university of medical sciences, faculty of pharmacy, pharmaceutical sciences research center, department of phytopharmaceuticals (traditional pharmacy), iran
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پست الکترونیکی
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mohaghegh@sums.ac.ir
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Quality control of Rosa × damascena flowers from Layzangan of Fars province in Iran
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Authors
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Mosleh Ghazaleh ,Badr Parmis ,Azadi Amir ,Afsari Sardari Forough ,Iraji Aida ,Khademian Sedigheh ,Abolhassanzadeh Zohreh ,Mohagheghzadeh Abdolali
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Abstract
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Background: Damask rose (DR) with the scientific name of Rosa × damascena Herrm. is a popular medicinal plant belonging to the Rosaceae family. According to the literature, DR cultivation was originally developed in the Layzangan valley, Darab region of Fars province in Iran. Objective: In this study, quality control and standardization of Layazangan DR flowers were investigated. Methods: Histological, physicochemical, and heavy metal analysis as well as the chromatographic fingerprints including GCMS and HPLC analysis were studied. Results: According to the microscopic observations, secretory and simple trichromes in sepal, rosette crystals in ovule, and the tricolporate shape of pollen grain were found to be the major characteristics in DR flowers. The results of heavy metal analysis revealed that the amounts of zinc (82.5 ppm), copper (4.02 ppm), lead (0.11 ppm), and cadmium (not detected) were less than maximum permitted amounts. The essential oil of fresh petals was obtained by hydrodistillation and was analyzed by GCMS method. Twentythree components were identified representing 98.66 % of the total essential oil composition. Citronellol (41.44 %), nonadecane (16.44 %), and heneicosane (10.58 %) were the main components. According to HPLC analysis results, quercetin was determined as 7300.5 mu;g/g dried DR petals. Conclusion: The essential oil obtained from Layzangan DR is rich in citronellol compound. On the other hand, the diagnostic characteristics presented in this study can help in better quality control and standardization of DR samples. It could also provide information for the authentication of relevant undeclared samples as well as the detection of adulterated materials.
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Keywords
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Damask rose ,Histological analysis ,GC-MS ,HPLC
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