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enhancement of taxol production by applying amino acid complex along with chitosan in suspension culture of taxus baccata l.
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نویسنده
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amirkavei najafabadi bahar ,qavami nasrin ,ebrahimi mohammad ali ,ebrahimi peyman ,zarinpanjeh nasim
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منبع
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journal of medicinal plants - 2020 - دوره : 19 - شماره : 76 - صفحه:99 -109
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چکیده
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Background: taxol (paclitaxel) has been highly recommended to control and treat a wide range of cancers. taxus baccata l. is primary and natural origin of taxol. objective: due to severe restriction and prohibition of cutting t. baccata, the alternative way for taxol production is to apply in vitro culture method which was implemented at the current study. here, for the first time, the effect of amino acid complex as elicitor along with chitosan in cell suspension culture oft. baccata for enhancing taxol production was studied. methods: young leaves of t. baccata as explants were cultured in different callus induction treatments. at the next step, the calli from the best callus induction treatment were transferred to cell suspension cultures containing chitosan and amino acid complex alone or in combination with each other. taxol content in treatments were measured by hplc. results: ms medium supplemented with 2,4d at 2 mg/l and naa at 1 mg/l proved to be the best treatment of callus induction (100 %), fresh weight (495 mg) and dry weight (272 mg) of calli. also, hplc analysis confirmed the maximum production of taxol (1.96 mg/g) in ms medium having 2 ml/l amino acids complex with 10 mg/l chitosan. conclusion: applying amino acid complex as elicitor with chitosan is suggested for enhancing taxol production in cell suspension culture of t. baccata.
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کلیدواژه
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cell culture ,elicitor ,medicinal plants ,secondary metabolites ,taxus baccata l.
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آدرس
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payam noor university, department of agricultural biotechnology, iran, acecr, institute of medicinal plants, medicinal plants research center, iran, payam noor university, department of agricultural biotechnology, iran, islamic azad university science and research branch, agricultural sciences and food industries faculty, iran, acecr, institute of medicinal plants, medicinal plants research center, iran
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پست الکترونیکی
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zarinpanjeh@imp.ac.ir
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enhancement of taxol production by applying amino acid complex along with chitosan in suspension culture of taxus baccata l.
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Authors
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Amirkavei Najafabadi Bahar ,Qavami Nasrin ,Ebrahimi Mohammad Ali ,Ebrahimi Peyman ,Zarinpanjeh Nasim
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Abstract
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background: taxol (paclitaxel) has been highly recommended to control and treat a wide range of cancers. taxus baccata l. is primary and natural origin of taxol. objective: due to severe restriction and prohibition of cutting t. baccata, the alternative way for taxol production is to apply in vitro culture method which was implemented at the current study. here, for the first time, the effect of amino acid complex as elicitor along with chitosan in cell suspension culture oft. baccata for enhancing taxol production was studied. methods: young leaves of t. baccata as explants were cultured in different callus induction treatments. at the next step, the calli from the best callus induction treatment were transferred to cell suspension cultures containing chitosan and amino acid complex alone or in combination with each other. taxol content in treatments were measured by hplc. results: ms medium supplemented with 2,4d at 2 mg/l and naa at 1 mg/l proved to be the best treatment of callus induction (100 %), fresh weight (495 mg) and dry weight (272 mg) of calli. also, hplc analysis confirmed the maximum production of taxol (1.96 mg/g) in ms medium having 2 ml/l amino acids complex with 10 mg/l chitosan. conclusion: applying amino acid complex as elicitor with chitosan is suggested for enhancing taxol production in cell suspension culture of t. baccata.
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Keywords
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cell culture ,elicitor ,medicinal plants ,secondary metabolites ,taxus baccata l.
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