isolation and characterisation of anti-diabetic pharmacological activities of phytoestrogens

Background: isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. these plant derived compounds have been shown to play a beneficial role in obesity and diabetes.objective: in this study the impact of these phytoestrogens on glucose uptake in hepg2 cell were compared.methods: glucose uptake measurement was performed using 2(n(7nitrobenzin2oxa1, 3 diazol4yl) amino)2deoxyglucose (2nbdg) and omega fluostar plate reader. incubation of cells (104/ml, in 24 well plate at 37 °c in 5% co2 / air) with three phytoestrogens at concentration of 104 m to 109m in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested.results: the data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 micro;m, which stimulated uptake about threefold compared with control. longer treatments with 104m to 106 m genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. a short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrationsconclusion: overall hepg2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. there was significant difference in glucose uptake between short and long term treatments, as indicated by twoway anova.