changes of tropane alkaloids in black henbane (hyoscyamus niger l.) in response to different types of nitrogenous fertilizers

Background: since alkaloids are nitrogenous compounds, the availability of nitrogen (n) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. nitrogen has been found to increase the content of alkaloids in some of the medicinal plants.objective: the aim of this study was to compare the effects of nitrogen biofertilizers, conventional nitrogen fertilizer, and nitrogen nanochelate fertilizer on the content of tropane alkaloids in hyoscyamus niger l.methods: this experiment was conducted in a randomized complete block design (rcbd) with 8 treatments, and 3 replications. the experimental treatments included 2 l.h1 nitroxin, 1 kg.h1 azotobacter, and 10 t.h1 vermicompost each as biofertilizer, 150 kg.h1 urea as a conventional chemical fertilizer of nitrogen, and nitrogen nanochelate fertilizer with three amounts of 3, 5, and 7 percent, and control treatment (without nitrogen fertilizer).results: the results showed that the effects of biofertilizers, conventional nitrogen fertilizer, and nitrogen nanochelate fertilizer were significant on the scopolamine and hyoscyamine content in black henbane at the level of 1%. the highest content of hyoscyamine (hyo) was related to the treatments of 5% per hectare nitrogen nanochelate, and the maximum amount of scopolamine (sco) was observed in the treatments of 3 and 5% nitrogen nanochelate fertilizers. on the other hand, the lowest values of hyoscyamine and scopolamine were related to the treatment of 7% nitrogen nanochelate fertilizer, and nitroxin treatment, respectively.conclusion: generally, the highest yield of alkaloids content in black henbane were obtained by application of 3 and 5% nitrogen nanochelate fertilizers.