Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves

Background: woodfordia fruticosa is used traditionally as antidysenteric compound for fodderanimals. stem and flower extracts are previously flourished for their antibacterial activity.leaves are also equally important, which are preliminary screened for their bioactivecomponents in present study.objective: evaluation of antibacterial activity of leaves of w. fruticosa and detection of theiractive compound.methods: different solvent i.e. petroleum ether, chloroform, diethyl ether and acetone extractswere studied against four bacterial strains using disc diffusion assay. most potenz extract waschromatogram on preparative tlc. further individual separated spots were back checked fortheir activity. probable constituents of most active fraction were detected on gc-ms.results: all four extracts exhibited antibacterial activity. the acetone extract showed highestinhibition against bacillus subtilis ncim 2921. the 80μg and 120 μg of acetone extract exhibitcomparable antimicrobial activity against the four different bacteria studied. amongst the fourdifferent spots on tlc chromatogram three of them had activity against b. subtilis ncim 2921.the most active fraction was collected by preparative tlc and used for gc-ms, which exhibitedtwo peaks.conclusion: extracts of petroleum ether, chloroform, diethyl ether and acetone were found tobe effective against all the srains studied. the fraction of acetone extract, which is more active,constitutes two different compounds, in which both or one of them is responsible for antibacterialactivity.