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Effects of Bothrops moojeni venom on Leishmania amazonensis promastigote forms
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نویسنده
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castilhos p. ,pereira c.g. ,silva a.l.n. ,napolitano d.r. ,oliveira f. ,souza m.a.
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منبع
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journal of venomous animals and toxins including tropical diseases - 2011 - دوره : 17 - شماره : 2 - صفحه:150 -158
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چکیده
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In the present work,the effect of bothrops moojeni venom fractions on leishmania promastigotes was evaluated. the snake venom was fractionated into five protein fractions (e1 to e5),by ion exclusion chromatography,that were used to treat leishmania amazonensis and leishmania braziliensis promastigote forms whereas the viability and nitric oxide production were evaluated. it was observed that e5 venom fraction strongly inhibited leishmania amazonensis nitric oxide production,while in leishmania braziliensis the nitric oxide production was enhanced in all doses. bothrops moojeni crude venom reduced the viability of both parasites in a dose-dependent manner and a peptide of 64 kda was apparently degraded. bothrops moojeni e5 venom fraction only reduced the viability and nitric oxide production of leishmania amazonensis and no protein degradation was observed. thus,these results suggest that bothrops moojeni e5 venom fraction may offer components with a promising antileishmanial therapeutic application. © cevap 2011.
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کلیدواژه
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Bothrops moojeni; Leishmania spp.; Nitric oxide; Snake venom
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آدرس
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laboratory of molecular biology,institute of biomedical sciences,federal university of uberlândia,uberlândia,minas,gerais state,brazil,laboratory of biophysics,federal university of uberlândia,uberlândia,minas,gerais state, Brazil, laboratory of molecular biology,institute of biomedical sciences,federal university of uberlândia,uberlândia,minas,gerais state, Brazil, laboratory of molecular biology,institute of biomedical sciences,federal university of uberlândia,uberlândia,minas,gerais state, Brazil, laboratory of biophysics,federal university of uberlândia,uberlândia,minas,gerais state, Brazil, laboratory of biophysics,federal university of uberlândia,uberlândia,minas,gerais state, Brazil, laboratory of molecular biology,institute of biomedical sciences,federal university of uberlândia,uberlândia,minas,gerais state, Brazil
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Authors
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