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Hemolytic activity of venom from crown-of-thorns starfish Acanthaster planci spines
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نویسنده
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lee c.-c. ,tsai w.-s. ,hsieh h.j. ,hwang d.-f.
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منبع
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journal of venomous animals and toxins including tropical diseases - 2013 - دوره : 19 - شماره : 1
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چکیده
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Background: the crown-of-thorns starfish acanthaster planci is a venomous species from taiwan whose venom provokes strong hemolytic activity. to understand the hemolytic properties of a. planci venom,samples were collected from a. planci spines in the penghu islands,dialyzed with distilled water,and lyophilized into a. planci spine venom (asv) powder. results: both crude venom and asv cause 50% hemolysis at a concentration of 20 μg/ml. the highest hemolytic activity of asv was measured at ph 7.0-7.4; asv-dependent hemolysis was sharply reduced when the ph was lower than 3 or greater than 8. there was almost no hemolytic activity when the cu2+ concentration was increased to 10 mm. furthermore,incubation at 100°c for 30 to 60 minutes sharply decreased the hemolytic activity of asv. after treatment with the protease α-chymotrypsin,the glycoside hydrolase cellulase,and the membrane component cholesterin,the hemolytic activity of asv was significantly inhibited. conclusions: the results of this study provide fundamental information about a. planci spine venom. the hemolytic activity was affected by ph,temperature,metal ions,edta,cholesterin,proteases,and glycoside hydrolases. asv hemolysis was inhibited by cu2+,cholesterin,α-chymotrypsin,and cellulose,factors that might prevent the hemolytic activity of venom and provide the medical treatment for sting. © 2013 lee et al.; licensee biomed central ltd.
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کلیدواژه
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Crown-of-thorns starfish; Hemolysis; Spine; Venoms
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آدرس
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national taiwan ocean university,department of food science and center of excellence for marine bioenvironment and biotechnology, Taiwan, fisheries research institute,penghu marine biology research center,council of agriculture,magong,penghu, Taiwan, asia university,department of health and nutrition biotechnology, Taiwan, national taiwan ocean university,department of food science and center of excellence for marine bioenvironment and biotechnology,taiwan,asia university,department of health and nutrition biotechnology, Taiwan
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Authors
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