Comparative analysis of the venom proteome of four important Malaysian snake species

Background: naja kaouthia,ophiophagus hannah,bungarus fasciatusandcalloselasma rhodostomaare four venomous snakes indigenous to malaysia. in the present study,their proteomic profile by two-dimensional gel electrophoresis (2-de) have been separated and compared.results: the 2-de of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. a total of 63 proteins were identified in the four species:c. rhodostoma- 26,n. kaouthia- 16,o. hannah- 15 andb. fasciatus- 6.conclusions: despite the identifications of major proteins in the four snake species,a large number of protein spots from the 2-de were unidentified even though the spots displayed high-quality maldi-tof-ms spectra. those identified included phospholipase a2proteins in all four venoms,long neurotoxins in both cobra species and thec. rhodostomavenom found with the most varied types of peptidases,i.e. metalloproteinase kistomin,halystase and l-amino acid oxidase. © 2014 vejayan et al.