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Antimycobacterial and cytotoxicity activity of microcystins
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نویسنده
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ramos d.f. ,matthiensen a. ,colvara w. ,de votto a.p.s. ,trindade g.s. ,da silva p.e.a. ,yunes j.s.
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منبع
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journal of venomous animals and toxins including tropical diseases - 2015 - دوره : 21 - شماره : 1
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چکیده
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Background: the present work aimed to evaluate the antimycobacterial activity and cytotoxicity ofmicrocystis aeruginosatoxins,the mc-lr variant and purified extract of [d-leu1] microcystin-lr.methods: the antimicrobial activity ofm. aeruginosaextract and microcystin was evaluated by resazurin microtiter assay againstmycobacterium tuberculosis,m. terrae,m. chelonaeandm. kansasii. the cytotoxicity assay was performed by trypan blue exclusion against the htc cell line.results: antimicrobial activity was observed in the hexanic extract ofm. aeruginosa(rst 9501 strain) againstm. tuberculosis,including sensitive and resistant strains with minimal inhibitory concentrations (mic) between 1.93 μm and 0.06 μm. the high activity ofm. aeruginosahexanic extract could be attributed to the major presence of the toxins mc-lr and [d-leu1] mc-lr that showed activity at mic between 53 and 0.42 μm against tested mycobacterial strains. even at the highest concentration tested,no toxicity ofm. aeruginosaextracts was identified against htc cells.conclusions: these preliminary results suggest that [d-leu1] mc-lr is a promising candidate for the development of a new antimycobacterial agent. © 2015 ramos et al.
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کلیدواژه
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Antimycobacterial agents; Cytotoxic activity; Microcystins; Mycobacteria
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آدرس
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research center in medical microbiology,federal university of rio grande (furg),rio grande,rio grande do sul state, Brazil, brazilian corporation of agricultural research (embrapa),concórdia,santa catarina state, Brazil, school of chemistry and food,federal university of rio grande (furg),rio grande,rio grande do sul state, Brazil, graduation program in physiological sciences,institute of biological sciences,federal university of rio grande (furg),rio grande,rio grande do sul state, Brazil, graduation program in physiological sciences,institute of biological sciences,federal university of rio grande (furg),rio grande,rio grande do sul state, Brazil, research center in medical microbiology,federal university of rio grande (furg),rio grande,rio grande do sul state, Brazil, laboratory of cyanobacteria and phycotoxins,institute of oceanography,federal university of rio grande (furg),rio grande,rio grande do sul state, Brazil
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Authors
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