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Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom
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نویسنده
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costal-oliveira f. ,guerra-duarte c. ,oliveira m.s. ,castro k.l.p. ,lopes-de-sousa l. ,lara a. ,gomes e.r.m. ,bonilla c. ,guatimosim s. ,melo m.m. ,chávez-olórtegui c.
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منبع
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journal of venomous animals and toxins including tropical diseases - 2017 - دوره : 23 - شماره : 1
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چکیده
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Background: hadruroides lunatus is the most abundant scorpion species in the peruvian central coast,where most of the accidents involving humans are registered. in spite of its prevalence,there are only very few studies on h. lunatus envenomation. the aim of the present study was to analyze the cardiorespiratory alterations caused by h. lunatus envenomation in rodents. methods: wistar rats injected with h. lunatus scorpion venom were submitted to electrocardiography. after euthanasia,rat lungs were collected and histopathologically analyzed. mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. data were analyzed by anova or student's t-test. the significance level was set at p < 0.05. results: it was observed that h. lunatus venom increased heart rate and caused arrhythmia,thereby impairing the heart functioning. lungs of envenomed animals showed significant alterations,such as diffuse hemorrhage. in addition,immunofluorescence showed that h. lunatus venom was capable of binding to cardiomyocytes. furthermore,mouse ventricular cardiomyocytes incubated with h. lunatus venom showed a significant decrease in calcium transient,confirming that h. lunatus venom exerts a toxic effect on heart. conclusion: our results showed that h. lunatus venom is capable of inducing cardiorespiratory alterations,a typical systemic effect of scorpionism,stressing the importance of medical monitoring in envenomation cases. © 2016 the author(s).
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کلیدواژه
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Calcium transient; Cardiorespiratory alterations; Electrocardiography; Hadruroides lunatus venom; Immunofluorescence
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آدرس
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federal university of minas gerais,department of biochemistry and immunology,institute of biological sciences,cp: 486,belo horizonte,mg,cep: 31270-901, Brazil, federal university of minas gerais,department of biochemistry and immunology,institute of biological sciences,cp: 486,belo horizonte,mg,cep: 31270-901, Brazil, college of veterinary medicine,federal university of minas gerais,belo horizonte mg, Brazil, federal university of minas gerais,department of biochemistry and immunology,institute of biological sciences,cp: 486,belo horizonte,mg,cep: 31270-901, Brazil, federal university of minas gerais,department of biochemistry and immunology,institute of biological sciences,cp: 486,belo horizonte,mg,cep: 31270-901, Brazil, federal university of minas gerais,department of physiology and biophysics,institute of biological sciences,belo horizonte mg, Brazil, federal university of minas gerais,department of physiology and biophysics,institute of biological sciences,belo horizonte mg, Brazil, universidad nacional mayor de san marcos y universidad científica del sur,instituto nacional de salud,lima, Peru, federal university of minas gerais,department of physiology and biophysics,institute of biological sciences,belo horizonte mg, Brazil, college of veterinary medicine,federal university of minas gerais,belo horizonte mg, Brazil, federal university of minas gerais,department of biochemistry and immunology,institute of biological sciences,cp: 486,belo horizonte,mg,cep: 31270-901, Brazil
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Authors
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