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Protein-species quantitative venomics: Looking through a crystal ball
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نویسنده
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calvete j.j. ,petras d. ,calderón-celis f. ,lomonte b. ,encinar j.r. ,sanz-medel a.
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منبع
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journal of venomous animals and toxins including tropical diseases - 2017 - دوره : 23 - شماره : 1
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چکیده
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In this paper we discuss recent significant developments in the field of venom research,specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom,and the absolute quantification of the venom proteins (the term protein species is used here to refer to all the different molecular forms in which a protein can be found. please consult the special issue of jornal of proteomics towards deciphering proteomes via the proteoform,protein speciation,moonlighting and protein code concepts published in 2016,vol. 134,pages 1-202). challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out comprehensive quantitative analysis of all toxins present in a venom. this short essay reflects the authors' view of the immediate future in this direction for the proteomic analysis of venoms,particularly of snakes. © 2017 the author(s).
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کلیدواژه
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Absolute quantification; Inductively coupled plasma mass spectrometry; Protein species-resolved venomics; Snake venomics; Top-down proteomics; Top-down venomics
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آدرس
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instituto de biomedicina de valencia,c.s.i.c,structural and functional venomics laboratory,jaime roig 11,valencia,46010, Spain, university of california-san diego,skaggs school of pharmacy and pharmaceutical sciences,la jolla,ca, United States, university of oviedo,department of physical and analytical chemistry,oviedo, Spain, universidad de costa rica,instituto clodomiro picado,facultad de microbiología,san josé, Costa Rica, university of oviedo,department of physical and analytical chemistry,oviedo, Spain, university of oviedo,department of physical and analytical chemistry,oviedo, Spain
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Authors
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