In vitro propagation of khaya ivorensis from coppiced shoots

Khaya ivorensis,commonly known as african mahogany and belonging to the meliaceae family is a medium hardwood tree. a study was conducted to provide preliminary information regarding the utilization of coppiced shoots for in vitro micropropagating of k. ivorensis. coppiced shoots,which were approximately 30 40 cm long after two months were harvested and cut into 2-cm long segments with at least one axillary bud after the leaves had been removed. plant material manipulations from this step onwards were done under laminar flow hood in contamination-free conditions. immediately after disinfection,the explants were inoculated onto different basal media without any plant hormone for two weeks before being transferred onto the same media with the four concentrations of 6-benzylaminopurine (bap). shoot production data were analysed using statistical analysis system package (anova). least significant difference (lsd) test showed significant differences in mean number of shoots and leaves produced from different basal media and bap concentrations.