Development of qualitative and quantitative ELISA models for bovine brucellosis diagnosis [Si{dotless}gǧir brusellozis tani{dotless}si{dotless} için kalitatif ve kantitatif ELISA modellerinin geliştirilmesi]

In this study,a qualitative and a quantitative elisa models (ielisa and qelisa,respectively) have been developed for screening and quantifying igg isotype anti-brucella abortus lps antibody. for developing high sensitive and specific elisa models,the nature and concentration of blocking-diluting reagents have been found very critical since milk components such as casein and particularly β-lactoglobulin,but not α-lactalbumin,strongly inhibited the anti-lps antibody detection by an unknown mechanism. the ielisa as well as qelisa models have been developed and validated by oie reference sera and well-known field sera evaluated with rbt,crt and celisa. the results demonstrated that both elisa models,ielisa in large-scale screening and qelisa in standard determination of anti-lps antibody concentration,developed in this study can be included as valuable and effective immunodiagnostic tools in brucellosis monitoring-eradication and vaccination surveillance programs in endemic countries.